Product Info Summary
SKU: | A02516-3 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-SPAK/STK39 Antibody Picoband™
SKU/Catalog Number
A02516-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SPAK/STK39 Antibody Picoband™ catalog # A02516-3. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SPAK/STK39 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02516-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SPAK/STK39 recombinant protein (Position: Q338-S545).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02516-3 is reactive to STK39 in Human, Mouse, Rat
Applications
A02516-3 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
65-70 kDa
Calculated molecular weight
59.474kDa
Background of STK39
STE20/SPS1-related proline-alanine-rich protein kinase is an enzyme that in humans is encoded by the STK39 gene. This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IF analysis of SPAK/STK39 using anti-SPAK/STK39 antibody (A02516-3).
SPAK/STK39 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-SPAK/STK39 Antibody (A02516-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 2. Western blot analysis of SPAK/STK39 using anti-SPAK/STK39 antibody (A02516-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human DAUDI whole cell lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPAK/STK39 antigen affinity purified polyclonal antibody (Catalog # A02516-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPAK/STK39 at approximately 65-70KD. The expected band size for SPAK/STK39 is at 65-70KD.
Click image to see more details
Figure 3. Flow Cytometry analysis of HL-60 cells using anti-SPAK/STK39 antibody (A02516-3).
Overlay histogram showing HL-60 cells stained with A02516-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPAK/STK39 Antibody (A02516-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For STK39 (Source: Uniprot.org, NCBI)
Gene Name
STK39
Full Name
STE20/SPS1-related proline-alanine-rich protein kinase
Weight
59.474kDa
Superfamily
protein kinase superfamily
Alternative Names
DCHTsmall intestine SPAK-like kinase; DKFZp686K05124; EC 2.7.11; EC 2.7.11.1; PASK; proline-alanine-rich STE20-related kinase; serine threonine kinase 39; Serine/threonine-protein kinase 39; SPAKserine threonine kinase 39 (STE20/SPS1 homolog, yeast); STE20/SPS1 homolog; STE20/SPS1-related proline-alanine-rich protein kinase; Ste20-like protein kinase; Ste-20-related kinase STK39 DCHT, PASK, SPAK serine/threonine kinase 39 STE20/SPS1-related proline-alanine-rich protein kinase|STE20/SPS1 homolog|Ste20-like protein kinase|proline-alanine-rich STE20-related kinase|serine threonine kinase 39 (STE20/SPS1 homolog, yeast)|serine/threonine-protein kinase 39|small intestine SPAK-like kinase|ste-20-related kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STK39, check out the STK39 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STK39: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SPAK/STK39 Antibody Picoband™ (A02516-3)
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